Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
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چکیده
منابع مشابه
Separation of Binding Protein of Celangulin V from the Midgut of Mythimna separata Walker by Affinity Chromatography
Celangulin V, an insecticidal compound isolated from the root bark of Chinese bittersweet, can affect the digestive system of insects. However, the mechanism of how Celangulin V induces a series of symptoms is still unknown. In this study, affinity chromatography was conducted through coupling of Celangulin V-6-aminoacetic acid ester to the CNBr-activated Sepharose 4B. SDS-PAGE was used to anal...
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Effects of Periplocoside P from Periploca sepium on the Midgut Transmembrane Potential of Mythimna separata Larvae
Periplocoside P (PSP) isolated from the root bark of Periploca sepium contains a pregnane glycoside skeleton and possesses high insecticidal properties. Preliminary studies indicated that PSP disrupts epithelial functions in the midgut of lepidopteran larvae. In the present study, we examined the effects of PSP on the apical and basolateral membrane voltages, Va and Vbl, respectively, of cells ...
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ژورنال
عنوان ژورنال: Toxins
سال: 2015
ISSN: 2072-6651
DOI: 10.3390/toxins7051738